Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii × nigra

Late-embryogenesis-abundant proteins (LEA) typically accumulate to high levels during seed dehydration and have an important role in the plant response abiotic stresses. Transgenic hybrid poplars of 9-year-old TaLEA lines showed adaptation drought saline–alkali conditions. The exogenous gene was expressed normally, T-DNA insertion sites were identified on different chromosomes. Improving survival rate afforested species is essential for forest production areas. could provide potential genetic materials semi-arid We investigated growth stability adaptability transgenic Populus simonii × nigra, as well position genes. poplar field trials carried out using a multi-site joint analysis. Molecular characteristics by genome resequencing. XL-9, XL-7, XL-13 preferred consistently highest most stable across three testing sites. integrated into chromosome normally XL-1, XL-9 after 9 years. are subsequent tests. can be selected 2 years experiments. results this study will further guidance trees future production.